were incubated with horseradish per

were incubated with horseradish peroxidase (HRP)-conjugated
secondary antibodies. Immunoreactive sites in the sections
were visualized in brown after staining with diaminobenzidine
and mounting for observation using bright-field microscopy
(Axioskop 2 plus, Zeiss, Germany). The negative controls
were incubated with a solution devoid of any primary
antibody.
To compare ratios of seminiferous tubules with positive
Pdcd4 signals between groups, over 1600 seminiferous tubules
were counted for each mouse with three biological replications.
And for seminiferous tubules with positive PDCD4
signals (over 40), the ratio of testicular cells with positive
PDCD4 signal was also calculated and compared.

Results (English) 2:

were incubated with Horseradish peroxidase (HRP) -conjugated
Secondary antibodies. Immunoreactive Sites in The sections
were visualized in Brown After staining with diaminobenzidine
and mounting for Bright-Field Observation using microscopy
(Axioskop 2 Plus, Zeiss, Germany). The Negative Controls
were incubated with a Solution devoid of any Primary
antibody.
To Compare ratios of seminiferous tubules with positive
Pdcd4 Signals between groups, over 1,600th seminiferous tubules
were counted for each Mouse with Three Biological replications.
And for seminiferous tubules with positive PDCD4
Signals (. over 40), The ratio of testicular cells with positive
PDCD4 Signal was also calculated and compared.

Results (English) 3:

Were incubated with horseradish peroxidase (HRP) - conjugated
secondary antibodies. Immunoreactive sites in the sections
were. Visualized in brown after staining with diaminobenzidine
and mounting for observation using bright-field microscopy
(Axioskop, 2 plus. Zeiss, the Germany). The negative controls
were incubated with a solution devoid of any antibody primary
.
.To compare ratios of seminiferous tubules with positive
Pdcd4 signals, between groups over 1600 seminiferous tubules
were. Counted for each mouse with three biological replications.
And for seminiferous tubules with positive PDCD4
signals (over. 40), the ratio of testicular cells with positive
PDCD4 signal was also calculated and compared.