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Were soaked in 3% HCl to separate minerals and baked at a temperature was 60 ° C soaked in 5% NaOH to, separate protein. Soaked in 5% KMnO4 to separate pigment and fatty acid. Then the pure, chitin was soaked in 50% NaOH at 100 ° C in order to. Separate acetyl group and adjust the pH neutral to chitosan.
for one night.
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