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The result of the peak flow rate profile, you will see how to increase concentration, absorbance values to add up, and from a comparison of peak flow rate profile for each found that the flow rate 0.5 ml/min is the widest since the peak flow is slow in the pipe is too long. On the contrary, the 1.5 ml/min flow rate, it is a substance with a narrow peak due to the faster flow. The flow rate 1.0 ml parts/min because the proper flow, so the peak is not narrow, and does not look too wide.
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