Free-floating sections (40
m) were

Free-floating sections (40
m) were washed in
0.1 M PBS, incubated in 0.1 M PBS containing 5% normal
donkey serum and 0.3% Triton X-100 for 1 h. They
were subsequently incubated overnight with Alexa
546-conjugated streptavidin antibody (1:500) in PBS
containing 2% normal donkey serum at 4◦C, washed
with PBS and then mounted sequentially in glass slides
using Vectashield. The mounted slices were evaluated
for fluorescence under settings for 546 nm emissions
on a confocal microscope (Olympus, USA).

Results (English) 2:

Floating free-Sections (40? M) were washed in
0.1 M PBS, incubated in 0.1 M PBS containing 5% Normal
Donkey serum and 0.3% Triton X-100 for 1 H. They
were subsequently incubated Overnight with Alexa
546-conjugated streptavidin antibody (1: 500) in PBS
containing 2% Normal Donkey serum at 4 ° C, washed
with PBS and then sequentially in Glass Slides mounted
using Vectashield. The mounted slices were evaluated
for fluorescence under 546 NM settings for emissions
on a confocal Microscope (Olympus, USA).

Results (English) 3:

Free-floating sections (40 m) were washed in.0.1 M PBS incubated in, 0.1 M PBS containing 5% normal.Donkey Serum and 0.3% Triton X-100 for 1 h. They.Were subsequently incubated overnight with Alexa.546-conjugated streptavidin antibody (1: 500) in PBS.Containing 2% normal Donkey Serum at 4, education level C washed.With PBS and then mounted sequentially in glass slides.Using Vectashield. The mounted slices were evaluated.For fluorescence under settings for 546 nm emissions.On a confocal microscope Olympus USA (,).