Recent concerns over the sustainabi

Recent concerns over the sustainability of petrochemical-based processes for production of desired
chemicals have fueled research into alternative modes of production. Metabolic engineering of microbial
cell factories such as Saccharomyces cerevisiae and Escherichia coli offers a sustainable and flexible
alternative for the production of various molecules. Acetyl-CoA is a key molecule in microbial central
carbon metabolism and is involved in a variety of cellular processes. In addition, it functions as a
precursor for many molecules of biotechnological relevance. Therefore, much interest exists in
engineering the metabolism around the acetyl-CoA pools in cells in order to increase product titers.
Here we provide an overview of the acetyl-CoA metabolism in eukaryotic and prokaryotic microbes
(with a focus on S. cerevisiae and E. coli), with an emphasis on reactions involved in the production and
consumption of acetyl-CoA. In addition, we review various strategies that have been used to increase
acetyl-CoA production in these microbes.

Results (English) 3:

最近的关注，在可持续发展的石化为基础的工艺生产的所需的，化学物质的研究，以替代的生产模式。酿酒酵母和大肠杆菌的微生物的细胞工厂的代谢工程提供了一个可持续的和灵活的替代生产的各种分子。乙酰辅酶A是一个重要的分子，在微生物中的中心碳代谢，并参与各种细胞过程。此外，它的功能对于生物技术相关的许多分子的
前兆。因此，在细胞内的乙酰辅酶A池的代谢，以提高产品的效价，多大兴趣存在。在这里，我们提供了一个概述，在真核生物和原核微生物的乙酰辅酶A的代谢（与一个重点，对酿酒酵母和大肠杆菌），在生产和消费的乙酰辅酶A的重点反应。此外，我们回顾了各种策略，已被用来增加在这些微生物中的乙酰辅酶A的生产。