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Primer design.Primer pairs were automatically designed using.PRIMER 0.5 (http: / / www-genome.wi.mit.edu / ftp / pub.Software / primer.0.5), with parameters essentially as described.By Chen.5 Primers were 18 - 34, nucleotides longDevoid of secondary structure or consecutive tracts of a.Single nucleotide a GC, content around 50% (Tm approximately.60 cream C) and preferably G - or C-rich at the 3 end.Sequences that provided no useful primer pairs were eliminated.From further consideration.
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