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Four different trials.Depending on different types of diets i.e. Trial-I (normal.Diet), trial-II (high cholesterol diet), trial-III (high sucrose.Diet) and trial-IV (high cholesterol + high sucrose diet).Were conducted. Therfore 120 rats, were divided in four.Groups of thirty each. In each trial rats were, further divided.Into three subgroups comprising of ten rats in each.Subgroup. Rats were placed in standard polypropylene.Cages with stainless steel top grill. Rats were acclimatized.By feeding basal diet (AIN-76A) for a period of one week.At the initiation of study some rats were sacrificed to get.Baseline values. The temperature (23 edge 2 ° C) and relative.Humidity (2005-2006) 55 5% were maintained throughout the experiment.With 12 hours light - dark period. Feed & drink.Intake was recorded daily whilst body weight on weekly.Basis throughout the experiment. Spilled diet and feces.Were also collected.
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