Probiotics are the healthy living b

Probiotics are the healthy living bacteria when administered in adequate amounts confers health benefits in the host. The main objective of present study was to screen the bacteria for potential probiotic characters and enzyme production. The probiotic characters like tolerance to low pH, bile salts, antibiotic sensitivity, hydrophobicity and auto-aggregation properties were evaluated. Among all isolates Lactobacillus fermentum and Lactobacillus sp G3_4_1TO2 showed maximum potential probiotic characters and produced amylase enzyme by observing the halo zone around the colonies with the diameter 0.9 mm and 1.23 mm. Lactobacillus sp G3_4_1TO2 produced maximum amylase when compared with Lb. fermen- tum. The protein yield was 55.4% with the specific activity of 88.9 U/mg and obtained 40.8% purification fold. The molecular weight of amylase enzyme determined by SDS PAGE was 95,000 Da. From the present study it was considered that Lactobacillus sp G3_4_1TO2 was a potential probiotic bacteria producing maximum amylase enzyme.! 2018 Production and hosting by Elsevier B.V. on behalf of Academy of Scientific Research & Technology. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-

Results (English) 1:

Probiotics are the healthy living bacteria when administered in adequate amounts confers health benefits in the host. The main objective of present study was to screen the bacteria for potential probiotic characters and enzyme production. The probiotic characters like tolerance to low pH, bile salts, antibiotic sensitivity, hydrophobicity and auto-aggregation properties were evaluated. Among all isolates Lactobacillus fermentum and Lactobacillus sp G3_4_1TO2 showed maximum potential probiotic characters and produced amylase enzyme by observing the halo zone around the colonies with the diameter 0.9 mm and 1.23 mm. Lactobacillus sp G3_4_1TO2 produced maximum amylase when compared with Lb. fermen- tum. The protein yield was 55.4% with the specific activity of 88.9 U / mg and obtained 40.8% purification fold. The molecular weight of amylase enzyme determined by SDS PAGE was 95,000 Da. From the present study it was considered that Lactobacillus sp G3_4_1TO2 was a potential probiotic bacteria producing maximum amylase enzyme.
! 2018 Production and hosting by Elsevier BV on behalf of Academy of Scientific Research & Technology. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-.

Results (English) 2:

Probiotics are the healthy living bacteria when administered in adequate amounts confers health benefits in the host. The main objective of present study is to screen the bacteria for potential probiotic characters and enzyme production. The probiotic characters like tolerance to low pH, bile salts, antibiotic sensitivity, hydrophobicity and Auto-aggregation properties were evaluated. Among all isolates Lactobacillus fermentum and Lactobacillus SP G3_4_1TO2 showed maximum potential probiotic characters and produced amylase enzyme by observing the halo zone around the colonies with the diameter 0.9 mm and 1.23 mm. Lactobacillus sp G3_4_1TO2 produced maximum amylase when compared with Lb. Fermen-tum. The protein yield is 55.4% with the specific activity of 88.9 U/mg and obtained 40.8% purification fold. The molecular weight of amylase enzyme determined by SDS PAGE was 95,000 Da. From The present study it is considered that Lactobacillus SP G3_4_1TO2 was a potential probiotic bacteria producing maximum amylase enzyme. 2018 Production and hosting by Elsevier B.V. on behalf of Academy of Scientific Research & Technology. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-

Results (English) 3:

Probiotics are a healthy living bacteria when it is managed in the health benefits of the host. The main purpose of this study is to screen potential biological characteristics and enzyme production. Biological characteristics, such as low pH resistance, salt resistance, biological sensitivity, hydrophilicity and self aggregation. The isolation efficiency of Lactobacillus, fermenting bacteria, Lactobacillus, sp-3-pu-4 and u-1to2 were evaluated. The results showed the greatest biological potential. Based on the observation of halophilic bacteria, the characteristics of halophilic bacteria and the way of enzyme production were studied. Lactobacillus sp-g3, you 4u-1to2 produce the largest amylase and the protein yield of LB fermentation is 55.4% s The specificity of 88.9.mg was determined and 40.8% purity was obtained. SDS PAGE is 9500-da from the current study that Lactobacillus is a potential biological agent. Bacteria produce the largest enzymes.
Elsevier. B.V School of behavioral science and technology, produced and hosted in 2018, is open Visit CC by-nc-nd license (HTTP) under article creativecommons.org account license from NC-NC