1042.3.4 Identification of selected

1042.3.4 Identification of selected thermotolerant yeaststrainsA total of 19 isolates of thermotolerant yeastcapable of producing ethanol at high temperatures weresubjected to identification by D1/D2 domain of 26s rDNAsequencing analysis (8). Figure 2 showed phylogeneticanalysis of nucleotide sequences of D1/D2 domain of 26srDNA gene of selected thermotolerant yeast strains andthose of related species from GenBank. Based on thephylogenetic analysis, the selected thermotolerant yeaststrains can be clustered into 3 groups. The yeast isolateS1-2 and S10-2 were closely related to Pichia kudriavzevii,while isolate S6-1, S10-1 and S11-3 were closely relatedto Issatchenkia orientalis (synonym; Candida krusei). Thelast group, i.e., isolate S11-2, S15-1, S20-1, S23-5, S28-1,28-2, S29-1, S29-2, S30-2, S30-3, S31-1, S32-1, S32-2and S33-1 were closely related to Candida tropicalis,respectively
There are several thermotolerant yeasts that havebeen previously reported as ethanol-producing strains, e.g.,K. marxianus (3), Pichia sp., Candida sp., and some of S.cerevisiae (17). In this study, three species were found,i.e., P. kudriavzevii, Iss. orientalis, and C. tropicalis. Itshould be also noticed from this finding that C. tropicaliswas a predominant thermotolerant yeast found in sugarcaneplantation and sugar factories. The yeast Candida sp. hasbeen found in various natural sources such as nectar ofHibiscus R Osa-sinensis and Ixora coccinea flowers (16)and maize (18)

Results (English) 2:

1042.3.4 Identification of selected thermotolerant yeaststrainsA total of 19 isolates of thermotolerant yeastcapable of producing ethanol at high temperatures weresubjected to identification by D1 / D2 domain of 26s rDNAsequencing analysis (8). Figure 2 showed phylogeneticanalysis of nucleotide sequences of D1 / D2 domain of 26srDNA gene of selected thermotolerant yeast strains andthose of related species from GenBank. Based on thephylogenetic analysis, the selected thermotolerant yeaststrains can be clustered into 3 groups. The yeast isolateS1-2 and S10-2 were closely related to Pichia kudriavzevii, while isolate S6-1, S10-1 and S11-3 were closely relatedto Issatchenkia orientalis (synonym; Candida krusei). Thelast group, ie, isolate S11-2, S15-1, S20-1, S23-5, S28-1,28-2, S29-1, S29-2, S30-2, S30-3, S31-1,. S32-1, S33-1 S32-2and were closely related to Candida tropicalis, respectively
Thermotolerant There are several yeasts that havebeen previously reported as ethanol-producing strains, eg, K. marxianus (3), Pichia sp., Candida sp., and some of S.cerevisiae (17). In this study, three species were found, ie, P. kudriavzevii, Iss. orientalis, and C. tropicalis. Itshould be also noticed from this finding that C. tropicaliswas a predominant thermotolerant yeast found in sugarcaneplantation and sugar factories. The yeast Candida sp. hasbeen found in various natural sources such as nectar ofHibiscus R Osa-sinensis and Ixora coccinea flowers (16) and maize (18).