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Laboratory laboratories are studied in virus detection topics with technical Nested reverse-transcription (RT)-PCR, Gel Electrophoresis, Real time RT-PCR and cell culture C6/36 cell, Vero cell, which techniques in a breeding cell. Step 1 Prepare cells from the bodybuilding cells, and then check the appearance of the cell. The Vero cell line has a similar type. The C6/36 cell line is characterized by the round cells. Thick lined cells, step 2, Subculture cells, 3 cell count, 4 Increasing the number of viruses by using Herpes simplex virus type 2 bodybuilders in Vero cell, see Cytopathic effects (CPE) appearance, Focal, degeneration in the monolayer layer of the host cell and the resulting host cell expands as the round style RI the exact shape and step 5 to collect the virus.
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